Journal: Frontiers in Microbiology

Article Title: Cytobacts: Abundant and Diverse Vertically Seed-Transmitted Cultivation-Recalcitrant Intracellular Bacteria Ubiquitous to Vascular Plants

doi: 10.3389/fmicb.2022.806222

Figure Lengend Snippet: Illumina MiSeq NGS-based bacterial diversity analysis on surface-sterilized shoot-tip tissues of four plant species. 16S rRNA gene V3–V4 region profiling of tomato, watermelon, periwinkle, and maize showing (A) distribution of OTUs under different phyla. (B) OTU shares common to different plant species. (C) Major classes and (D) major genera.

Article Snippet: After preliminary quantity and quality assessments, the embryo-derived DNA pooled from different fruit sources were pooled and submitted to M/s Xcelris Labs Ltd. (Ahmedabad, India) for 16S rRNA gene V3–V4 taxonomic profiling (sample IDs MG09 and MG10, respectively, for PF and AP kits).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Cytobacts: Abundant and Diverse Vertically Seed-Transmitted Cultivation-Recalcitrant Intracellular Bacteria Ubiquitous to Vascular Plants

doi: 10.3389/fmicb.2022.806222

Figure Lengend Snippet: PICRUSt functional analysis on 16S rRNA V3–V4 region profiles with Illumina MiSeq NGS platform on different plant species, tomato, watermelon, periwinkle/ Catharanthus , and maize. (A) KEGG Level 1 functional analysis and (B) COG Level 1 functions.

Article Snippet: After preliminary quantity and quality assessments, the embryo-derived DNA pooled from different fruit sources were pooled and submitted to M/s Xcelris Labs Ltd. (Ahmedabad, India) for 16S rRNA gene V3–V4 taxonomic profiling (sample IDs MG09 and MG10, respectively, for PF and AP kits).

Techniques: Functional Assay

Journal: Frontiers in Microbiology

Article Title: Cytobacts: Abundant and Diverse Vertically Seed-Transmitted Cultivation-Recalcitrant Intracellular Bacteria Ubiquitous to Vascular Plants

doi: 10.3389/fmicb.2022.806222

Figure Lengend Snippet: Illumina MiSeq NGS-based bacterial diversity analysis on watermelon seed-embryos with DNA extracted using PowerFood microbial DNA isolation kit (MG09) or Axygen DNA isolation kit (MG10). Distribution of phylogenetic groups based on 16S rRNA gene V3–V4 region taxonomic profiling at (A) phyla and (B) class levels. (C) Major families. (D) Major genera.

Article Snippet: After preliminary quantity and quality assessments, the embryo-derived DNA pooled from different fruit sources were pooled and submitted to M/s Xcelris Labs Ltd. (Ahmedabad, India) for 16S rRNA gene V3–V4 taxonomic profiling (sample IDs MG09 and MG10, respectively, for PF and AP kits).

Techniques: DNA Extraction

Journal: Frontiers in Microbiology

Article Title: Cytobacts: Abundant and Diverse Vertically Seed-Transmitted Cultivation-Recalcitrant Intracellular Bacteria Ubiquitous to Vascular Plants

doi: 10.3389/fmicb.2022.806222

Figure Lengend Snippet: Description of grapevine seed versus excised seed-embryo 16S rRNA V3–V4 amplicon-based taxonomic profiling. (A) Berry, seed, and excised seed-embryo in ‘Red Globe’ (‘RG’) and ‘Bangalore Blue’ (‘BB’). (B) Monitoring the fruit wash solutions for the microbial load by spotting on nutrient agar, the upper panel showing results after six sterile distilled water (SDW) washes, and the lower panel showing the outcome after one ethanol wash followed by six FDW washes. (C) Excised embryos from the seeds of ‘RG’ and ‘BB.’ (D) Monitoring seed-wash solutions. (E) Monitoring embryo-wash solutions. (F) Testing the seed-tissue homogenate for cultivable bacteria through SP-SDS of serial dilutions with no bacterial CFU for 2–4 weeks. (G) Seed-embryo homogenate tested for cultivable bacteria through SP-SDS showing bacterial CFU after 2–4 weeks.

Article Snippet: After preliminary quantity and quality assessments, the embryo-derived DNA pooled from different fruit sources were pooled and submitted to M/s Xcelris Labs Ltd. (Ahmedabad, India) for 16S rRNA gene V3–V4 taxonomic profiling (sample IDs MG09 and MG10, respectively, for PF and AP kits).

Techniques: Amplification, Sterility, Bacteria

Journal: Frontiers in Microbiology

Article Title: Cytobacts: Abundant and Diverse Vertically Seed-Transmitted Cultivation-Recalcitrant Intracellular Bacteria Ubiquitous to Vascular Plants

doi: 10.3389/fmicb.2022.806222

Figure Lengend Snippet: 16S rRNA metagene V3–V4 region profiling with Illumina MiSeq NGS platform on grapevine seeds and embryos. (A) Distribution of OTUs (%) under different phyla. (B) Major genera (OTU %) in the four samples.

Article Snippet: After preliminary quantity and quality assessments, the embryo-derived DNA pooled from different fruit sources were pooled and submitted to M/s Xcelris Labs Ltd. (Ahmedabad, India) for 16S rRNA gene V3–V4 taxonomic profiling (sample IDs MG09 and MG10, respectively, for PF and AP kits).

Techniques: